In vitro cytocompatibility and in vivo small animal studies for the translational mechanistic-biological-medical assessment of additively manufactured bioresorbable magnesium implants

The primary objective of SP-6 is to demonstrate the comprehensive cytocompatibility and biocompatibility of the implant structures and polymer coatings produced by the project partners. The resulting findings will be incorporated into the broader context of the characterization, modeling, and interdisciplinary evaluation of bioresorbable implants, as well as into the discussion of the results from the various working groups. The subproject can be broken down into five sequential objectives. The first objective is to demonstrate adequate basic cytocompatibility using fibroblast moncultures in order to rule out incompatible materials at an early stage. Building on this, an expanded cytokompatibility analysis is conducted through the co-cultivation of implant materials with additional cell types, as well as the establishment of multiculture models to facilitate a multimodal cytokompatibility analysis followed by a genotoxicity test. The most promising material-coating configurations will first be examined histopathologically and histomorphometrically in a cascade-like manner using the chorion-allantois membrane (CAM) model as part of biocompatibility analyses. Based on this, a further selection of suitable materials will be made for subsequent tissue compatibility and biodegradation studies in small animal models (subcutaneous, tibia). The ultimate goal is to conduct a comprehensive analysis and correlate the results of all subprojects.